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How To Make A Blood Smear. Components of a blood smear. Prick the ring finger of left hand with the help of prickling needle under all aseptic conditions discard the first two drOps of blood from skin puncture site. Now push the spreader across the slide. Common cause of a poor blood smear.
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In this video I quickly go over how to make a peripheral blood smear. The feathered edge the monolayer and the body and base of the smear. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. Spreader slide pushed across the slide in a jerky. Use clean high-quality microscope slides. Common cause of a poor blood smear.
Preparation of Blood Film.
Can occur with anemic patients Increase the angle of the slide you are using to smear with. Spreader slide pushed across the slide in a jerky. Push carefully along the length of the first slide to produce a thin smear of blood. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. Common cause of a poor blood smear. Collect blood in an EDTA tube and make the smears when back at the clinic.
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If the blood has been refrigerated allow it to return. Otherwise your smear may run right off of your horizontal slide and you will not have a nice feathered edge and your smear will be so thin you will have trouble finding enough cells. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. Aim for a blood droplet size of 4mm diameter. Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques.
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This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. Can occur with anemic patients Increase the angle of the slide you are using to smear with. Take one drop of venous or capillary blood. Spreader slide pushed across the slide in a jerky.
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Optimise spreading speed for length and a good feathered edge. Aim for a blood droplet size of 4mm diameter. Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques. The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide. Without delay place a spreader at an angle of 45 from the slide and move it back to make contact with the drop.
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Aim for a blood droplet size of 4mm diameter. Place a drop of blood on the patient slide near the frosted edge. Parts of Blood Smear A peripheral blood film consists of 3 parts. Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide. How to Make a Peripheral Blood Smear.
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The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide. Blood can also be taken from an EDTA sample. Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques. Components of a blood smear. Common cause of a poor blood smear.
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Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide. Do not touch the surface of the slide where the blood smear will be made. Specimen of blood can also be taken by venepuncture. Can occur with anemic patients Increase the angle of the slide you are using to smear with. Clean slides with 70 to 90 alcohol and allow to dry.
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Select the finger to puncture usually the middle or ring finger. Specimen of blood can also be taken by venepuncture. Place a small drop of blood or one side about 1-2 cm from one end. The tapering end of the blood film. How to perform a blood film in 3 easy stepsLearn more and visit httpswwwidexxcouk.
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I hope you can see the smear well enough in the video if not please leave a comment and. In infants puncture the heel. I hope you can see the smear well enough in the video if not please leave a comment and. Use clean high-quality microscope slides. Aim for a blood droplet size of 4mm diameter.
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The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide. Clean the area to be punctured with 70 alcohol. Otherwise your smear may run right off of your horizontal slide and you will not have a nice feathered edge and your smear will be so thin you will have trouble finding enough cells. In infants puncture the heel. Specimen of blood can also be taken by venepuncture.
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A well made blood smear consists of several areas. Drop of blood too large or too small 2. Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species.
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3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. Now push the spreader across the slide. Do not push the blood by having it ahead of the smearing slide. If the blood has been refrigerated allow it to return. Take one drop of venous or capillary blood.
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Now push the spreader across the slide. Take another clean slide and holding at an angle of about 45 deg touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action. This PULLS the blood across to make the smear. Now push the spreader across the slide. If the blood has been refrigerated allow it to return.
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Push carefully along the length of the first slide to produce a thin smear of blood. Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species. The slide should be clean. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. In this video I quickly go over how to make a peripheral blood smear.
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Parts of Blood Smear A peripheral blood film consists of 3 parts. Place a small drop of blood or one side about 1-2 cm from one end. They then tie a band above. The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide. Preparation of Blood Film.
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Do not touch the surface of the slide where the blood smear will be made. Push the edge of the pusher slide into the blood drop so that it spreads out completely along the pusher slide. A quick instructional video demonstrating. Do not touch the surface of the slide where the blood smear will be made. The drop and it spreads by capillary action along its edge.
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Put a small size of blood drop on each glass slide about half centimeter from its narrow edge on the right side. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. Take another clean slide and holding at an angle of about 45 deg touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action. Common cause of a poor blood smear. Aim for a blood droplet size of 4mm diameter.
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Put a small size of blood drop on each glass slide about half centimeter from its narrow edge on the right side. The tapering end of the blood film. This PULLS the blood across to make the smear. Parts of Blood Smear A peripheral blood film consists of 3 parts. Put a small size of blood drop on each glass slide about half centimeter from its narrow edge on the right side.
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Preparation of Blood Film. They then tie a band above. To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop. Aim for a blood droplet size of 4mm diameter. Select the finger to puncture usually the middle or ring finger.
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